Эффекты нейротрофина-3 и фактора роста нервов на морфологию, пролиферацию и гибель клеток глиомы С6: ФАРМАКОЛОГИЯ, КЛИНИЧЕСКАЯ ФАРМАКОЛОГИЯ

Александр Николаевич  Чернов; Екатерина Геннадьевна  Батоцыренова; Кристина Михайловна  Щепеткова; Елена Николаевна  Красникова; Вадим Анатольевич  Кашуро

Effects of neurotrophin-3 and nerve growth factor on the morphology, proliferation, and cell death of C6 glioma cells

PHARMACOLOGY, CLINICAL PHARMACOLOGY

Alexander N. Chernov Saint Petersburg State Pediatric Medical University; Institute of Experimental Medicine https://orcid.org/0000-0003-2464-7370
Ekaterina G. Batotsyrenova Saint Petersburg State Pediatric Medical University https://orcid.org/0000-0003-3827-4579
Kristina M. Shchepetkova Saint Petersburg State Pediatric Medical University https://orcid.org/0000-0002-8250-4178
Elena N. Krasnikova Saint Petersburg State Pediatric Medical University
Vadim A. Kashuro Saint Petersburg State Pediatric Medical University; Saint Petersburg State University https://orcid.org/0000-0002-7892-0048

Keywords:

C6 glioma nerve growth factor neurotrophin-3 proliferation differentiation morphology cell death глиома С6 фактор роста нервов нейротрофин-3 пролиферация дифференциация морфология гибель клеток

Abstract

Introduction. Due to the ineffectiveness of standard chemotherapy and radiotherapy for glioblastoma, there is a need to find new drugs that selectively and effectively target tumor cells while sparing healthy tissue cells. The aim of the study was the effects of neurotrophin-3 (NT-3) and β-form of nerve growth factor (β-NGF) for 3 and 7 days on the morphology, proliferation and death of rat C6 glioma cells. Materials and methods. C6 glioma cells were cultured in Petri dishes (250,000 cells/dish) in Eagle’s medium supplemented with 10% fetal bovine serum (FBS) at 37 °C and 5% CO2 for 7 days. Cells were treated with NT-3 (10 and 20 ng/ml) and β-NGF (100 and 200 ng/ml). Cell size and area, number, length, and ferning of processes were assessed using intravital digital microscopy with IM1000 and Image J (version 1.38r). The proliferation index and cell death rate (%) were also determined.
Results. β-NGF (100 and 200 ng/ml) exhibited pronounced cytostatic activity. This was demonstrated by a more than fourfold suppression of the proliferation index, a decrease in the degree of outgrowth ferning, and stimulation of cell death at both observation times. In contrast, the effect of NT-3 was demonstrated by a 2-3-fold increase in proliferation (days 3 and 7) and a sharp decrease in ferning and cell death. Conclusions. The results demonstrate the opposing effects of NT-3 and NGF on the morpho-functional profile of C6 cells.

Author Biographies

Alexander N. Chernov, Saint Petersburg State Pediatric Medical University; Institute of Experimental Medicine

Cand. Sci. (Biol.), Assistant, Dept. of Biological Chemistry, Saint Petersburg State Pediatric Medical University; Senior Researcher, Dept. of General Pathology and Pathological Physiology, Institute of Experimental Medicine

Ekaterina G. Batotsyrenova, Saint Petersburg State Pediatric Medical University

Dr. Sci. (Biol.), Associate Professor, Head, Dept. of General and Medicinal Chemistry named after prof. V.V. Khorunzhy; Professor, Dept. of Biological Chemistry

Kristina M. Shchepetkova, Saint Petersburg State Pediatric Medical University

Assistant, Dept. of Biological Chemistry

Elena N. Krasnikova, Saint Petersburg State Pediatric Medical University

Cand. Sci. (Chemistry), Associate Professor, Dept. of Biological Chemistry

Vadim A. Kashuro, Saint Petersburg State Pediatric Medical University; Saint Petersburg State University

Dr. Sci. (Med.), Associate Professor, Head, Dept. of Biological Chemistry, Saint Petersburg State Pediatric Medical University; Professor, Dept. of Maxillofacial Surgery and Surgical Dentistry, Saint Petersburg State University

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